Research Experience:

2004

6 months

Center for Neurologic Diseases, Harvard Medical School, Boston, USA

Position: Visiting Student

2001/2003

2 years

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Laboratory of Cellular and Molecular Neuroscience, The Rockefeller University, New York, USA

Position: Research Assistant

Ø      Project: development of a purification method of Casein Kinase 1 by affinity chromatography.

Research: research, with a two-hybrid screening method, of proteins interacting specifically with each Casein Kinase 1 isoform. Determination of the shortest interacting fragments, and their subcloning / expression / purification in bacteria system. Immobilization of these peptides on resin, and work on the conditions for a purification of the native enzyme by affinity chromatography. Purification efficiency test by kinase assay.

2001

9 months

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Laboratory of Cell Cycle, Station Biologique, Roscoff, France.

Position: Ingénieur d’Etudes

Project: eukaryotic expression of Casein Kinase 1 isoforms in the yeast Pichia pastoris.

1999/2001

1 year 1/2

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Laboratory of Genetic and Development, CNRS UMR 6061, Rennes, France

Position: Ingénieur d’Etudes

Ø      Project: functional study of Pol32, a gene involved in DNA replication and repair in the yeast S. cerevisiae.

1999

4 months

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1999                  Laboratory of Early Stage Development, CNRS UPR 41, Rennes, France

Position: student

Ø      Project: sub-cloning, expression and cell localization of Eg6, a protein involved in Xenopus laevis early stage development.